| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.047905 |
| Chromosome: | chromosome 14 |
| Location: | 2000571 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g621475 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCCTTTGGATCCAGCTGCTGCTGCAGT |
| Internal bar code: | GTCCGGTACAACATATCTCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 915 |
| LEAP-Seq percent confirming: | 96.7575 |
| LEAP-Seq n confirming: | 2238 |
| LEAP-Seq n nonconfirming: | 75 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGATAACGCGACTCAAGGC |
| Suggested primer 2: | GTGTGTCGTGAACGTTTTGG |