| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.047918 |
| Chromosome: | chromosome 15 |
| Location: | 460336 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g636550 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAACCCCGCCGCATTCAGGGTGCGTGCGT |
| Internal bar code: | GGCCCTGGTTTTTTATGGGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 832 |
| LEAP-Seq percent confirming: | 99.6555 |
| LEAP-Seq n confirming: | 2893 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCGGCATACAGTTTATTT |
| Suggested primer 2: | GCGAGAAGAACCAAACTTGC |