Insertion junction: LMJ.RY0402.047919_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.047919
Chromosome:chromosome 17
Location:2927434
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g719876 (1 of 4) PF12576 - Protein of unknown function (DUF3754) (DUF3754) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTACATTGATTACCATTGTCCCGACTACCG
Internal bar code:TAACTCATACCGGAGGTCGACT

Confirmation - LEAP-Seq

LEAP-Seq distance:94
LEAP-Seq percent confirming:95.1754
LEAP-Seq n confirming:217
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CACCTCTCTACCCCTCCCTC
Suggested primer 2:GTTTGCATCAAAGAGCAGCA