| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.047964 |
| Chromosome: | chromosome 2 |
| Location: | 5834335 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g111050 | AMT7,AMT1G | Ammonium transporter; (1 of 8) K03320 - ammonium transporter, Amt family (amt, AMT, MEP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAGGGCGCAGAGAGGGTGGGCCAGGGTT |
| Internal bar code: | CTGCGAACCACGTGGGTGTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 861 |
| LEAP-Seq percent confirming: | 99.7213 |
| LEAP-Seq n confirming: | 4293 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTTGTTGAATGGGTGAG |
| Suggested primer 2: | CGCAGGATAAGCAGAAGGTC |