Insertion junction: LMJ.RY0402.047981_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.047981
Chromosome:chromosome 8
Location:1986904
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g367900 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAGGAAGATACGAGCGCTGACCCGGAACA
Internal bar code:GAGGATTCACGTGGGTGGGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:804
LEAP-Seq percent confirming:98.3526
LEAP-Seq n confirming:2388
LEAP-Seq n nonconfirming:40
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:TGCATTTGGTTGCAAATTGT
Suggested primer 2:TGAATTAATGAAGCTGGGGC