| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.048012 |
| Chromosome: | chromosome 1 |
| Location: | 6230220 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044600 | (1 of 2) PF17047 - Synaptotagmin-like mitochondrial-lipid-binding domain (SMP_LBD) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCGCCGGGCAACAGCTTGCCCACGTCC |
| Internal bar code: | GAGGCAAGGAACTGGCATTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 148 |
| LEAP-Seq percent confirming: | 99.9334 |
| LEAP-Seq n confirming: | 1500 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCGGCTGTAGACTGCAC |
| Suggested primer 2: | ATCTCCCCTACTGGCAACCT |