| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.048019 |
| Chromosome: | chromosome 12 |
| Location: | 2587901 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g505150 | OPR52 | (1 of 781) IPR000104 - Antifreeze protein, type I; OctotricoPeptide Repeat protein 52 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTGGTGACCACAGCCACAACCGCGGCGT |
| Internal bar code: | GTGAAGTTGCTGGGTCGCCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 696 |
| LEAP-Seq percent confirming: | 97.7825 |
| LEAP-Seq n confirming: | 2293 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCTGTCTAGCTGACCACC |
| Suggested primer 2: | GCAAGTGAGGAATAGCCTGC |