| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.048023 |
| Chromosome: | chromosome 12 |
| Location: | 8349846 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549400 | (1 of 4) PF09366 - Protein of unknown function (DUF1997) (DUF1997) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTACCGGCGAACCAAACAGTGCTATGCAC |
| Internal bar code: | CGCGCTTGATGATCGATGGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 303 |
| LEAP-Seq percent confirming: | 71.224 |
| LEAP-Seq n confirming: | 11888 |
| LEAP-Seq n nonconfirming: | 4803 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTTGCGCTTTCGTGTAA |
| Suggested primer 2: | GATAGGTGAGTGGTTCGGGA |