| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.048046 |
| Chromosome: | chromosome 4 |
| Location: | 2418897 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g221301 | CYCD5 | D-type cyclin; (1 of 10) PTHR10177 - CYCLINE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGGTCGCGGCACAAAGCATGTCAAGCA |
| Internal bar code: | GCGGTTAGGTGTTATGGGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 864 |
| LEAP-Seq percent confirming: | 99.2403 |
| LEAP-Seq n confirming: | 1045 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATGCGCTGTATGAGCCAA |
| Suggested primer 2: | AGAGTAGGAAGGCGAAAGCC |