| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.048054 |
| Chromosome: | chromosome 12 |
| Location: | 40686 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g483550 | VPE1 | (1 of 1) K01369 - legumain (LGMN); Vacuolar processing enzyme | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATAACAGAAAGCCCGCCCAACCAAGAAT |
| Internal bar code: | AGAGCACTCGTAAGAGATGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 48 |
| LEAP-Seq percent confirming: | 14.8148 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 92 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATCGCTACAGCTACCGCT |
| Suggested primer 2: | TCGTGTTCTACAGCGACCAC |