| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.048064 |
| Chromosome: | chromosome 12 |
| Location: | 5606846 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532000 | (1 of 3) PF04359 - Protein of unknown function (DUF493) (DUF493) | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTACGTCAAGGTCACGAGGAAAGCCGGCG |
| Internal bar code: | TACTAACAGTACCGGGCACCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 816 |
| LEAP-Seq percent confirming: | 99.7224 |
| LEAP-Seq n confirming: | 3592 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTCCACGCTTTACCTGT |
| Suggested primer 2: | GTGTAGGTTGCACCTGCTGA |