Insertion junction: LMJ.RY0402.048076_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.048076
Chromosome:chromosome 16
Location:5182660
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g683100 (1 of 239) IPR016024 - Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACCGCAGGCACCGGTGCAGTTAATTGTT
Internal bar code:GGAGGAGGCCTGTCTGTTCTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:582
LEAP-Seq percent confirming:99.6522
LEAP-Seq n confirming:7450
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGCTCAAGGCCCAGAGTG
Suggested primer 2:GCTTTGATACCTGGGTGCAT