Insertion junction: LMJ.RY0402.048081_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.048081
Chromosome:chromosome 8
Location:4332398
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g381516 (1 of 1) K11168 - dehydrogenase/reductase SDR family member 12 (DHRS12) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAAATCCACTACCACATACACCGCCACC
Internal bar code:GCATTCAGCCGGGCTCGAAGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:582
LEAP-Seq percent confirming:99.4813
LEAP-Seq n confirming:4219
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTCCCACTCCTCTCACCT
Suggested primer 2:CCTCTCCAAATTCACCTCCA