Insertion junction: LMJ.RY0402.048087_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.048087
Chromosome:chromosome 3
Location:8842888
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g206817 (1 of 2) IPR001739//IPR016177 - Methyl-CpG DNA binding // DNA-binding domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGTCGCCGGGCAGCCTGGCGTGTGCTGG
Internal bar code:CTGCTAGGTCGCCCGCCTACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:406
LEAP-Seq percent confirming:99.7247
LEAP-Seq n confirming:2536
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ATTCAGTTACGCCGAGCTGT
Suggested primer 2:TGAGTTCAATGGGGAGGTTC