| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.048107 |
| Chromosome: | chromosome 12 |
| Location: | 8134887 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551353 | LAO1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGGACCAGCGGGTTCAGGCTGCGAGCAG |
| Internal bar code: | TATTCAATCGGTCAGTCCTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 683 |
| LEAP-Seq percent confirming: | 99.2271 |
| LEAP-Seq n confirming: | 2311 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCCTGATCATGGGTGAGT |
| Suggested primer 2: | GCAGCCTATCTACTGTGGGC |