| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.048140 |
| Chromosome: | chromosome 16 |
| Location: | 3525356 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677382 | (1 of 24) PTHR10641 - MYB-LIKE DNA-BINDING PROTEIN MYB | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCCAAATTAGCGGGCCTACGGTGCAGAG |
| Internal bar code: | TCTCTCCTGCAACCCTAGCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 838 |
| LEAP-Seq percent confirming: | 99.6522 |
| LEAP-Seq n confirming: | 3725 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTACAGCTGAAGGGGCAG |
| Suggested primer 2: | GCACATAGCTACAGGCACGA |