| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.048148 |
| Chromosome: | chromosome 4 |
| Location: | 2295151 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g220387 | CYP770A1 | (1 of 10) IPR001128//IPR002401 - Cytochrome P450 // Cytochrome P450, E-class, group I; Cytochrome P45. Previously annotated as CYP77A1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACAATTTCTCACCTGGCTTCCTTAATGC |
| Internal bar code: | CCTAGATCCATACTTCCAGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 918 |
| LEAP-Seq percent confirming: | 99.4966 |
| LEAP-Seq n confirming: | 4941 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCATCCTGATCTTGGGTTG |
| Suggested primer 2: | AGGTATGTACGGAGGCATCG |