| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.048157 |
| Chromosome: | chromosome 6 |
| Location: | 328054 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g251350 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGAATTGCGCGTAGTACTGCGGTACGG |
| Internal bar code: | ACGACGCATGACAGACCCTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 590 |
| LEAP-Seq percent confirming: | 98.5138 |
| LEAP-Seq n confirming: | 24261 |
| LEAP-Seq n nonconfirming: | 366 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGGTGTGTGGAGATGGG |
| Suggested primer 2: | CGTGTGCTTGTCAGCTGTG |