| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.048168 |
| Chromosome: | chromosome 9 |
| Location: | 2827712 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388750 | TEF21 | (1 of 1) PTHR11200:SF170 - PHOSPHOINOSITIDE PHOSPHATASE SAC1; Phosphoinositide phosphatase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCTTGCGAGCCTCCCTCCCCCGCAGTCT |
| Internal bar code: | ATACCGAGTGGGCCACCGTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 35 |
| LEAP-Seq percent confirming: | 98.8095 |
| LEAP-Seq n confirming: | 83 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGAACTCCTTCTCCACGG |
| Suggested primer 2: | CAGCTGCCCAACATCAAGTA |