| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.048184 |
| Chromosome: | chromosome 9 |
| Location: | 5154344 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399030 | HPD2 | (1 of 1) K00457 - 4-hydroxyphenylpyruvate dioxygenase (HPD, hppD); 4-hydroxyphenylpyruvate dioxygenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCTGTGGTTTGCTCCTGGTGACATGCAA |
| Internal bar code: | TCATGGTGCTAATCGTCGACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 985 |
| LEAP-Seq percent confirming: | 99.446 |
| LEAP-Seq n confirming: | 25850 |
| LEAP-Seq n nonconfirming: | 144 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGGAGCTGTGTCTATTTG |
| Suggested primer 2: | GTGTTGGATTGTATGCGACG |