Insertion junction: LMJ.RY0402.048193_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.048193
Chromosome:chromosome 9
Location:7529330
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g414150 GT90F42,GT90-42 GT90 family protein 42; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCAACACTCGTCAAATTGTGGGGCGCTTC
Internal bar code:ACTGGGTGGGGTTATACAGTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:774
LEAP-Seq percent confirming:61.7726
LEAP-Seq n confirming:690
LEAP-Seq n nonconfirming:427
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:ATGGGACAGTGGTAAGGCAG
Suggested primer 2:CTGTTTGATGACATGGACGG