| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.048208 |
| Chromosome: | chromosome 2 |
| Location: | 751069 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g078600 | RSP8 | (1 of 239) IPR016024 - Armadillo-type fold; Radial spoke protein 8 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCAGCTACAATTGTGCAGACGAGCCGGA |
| Internal bar code: | TGTCGTCCGACGAGTTAGCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 778 |
| LEAP-Seq percent confirming: | 99.0637 |
| LEAP-Seq n confirming: | 1058 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGAGAGCAAGGTCAACG |
| Suggested primer 2: | CACGAGTGCATCGAGGAGTA |