| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.048213 |
| Chromosome: | chromosome 1 |
| Location: | 4377269 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g029600 | (1 of 3) 1.11.2.3 - Plant seed peroxygenase / Soybean peroxygenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAAGGCAAAGTGCTGCTGCAGCGAGGTGA |
| Internal bar code: | GTTGATCGAATCTGGCGTAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 544 |
| LEAP-Seq percent confirming: | 99.6524 |
| LEAP-Seq n confirming: | 860 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCGACTACATGGACAAGC |
| Suggested primer 2: | TGTCTAACTGGAAGGGGGTG |