| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.048221 |
| Chromosome: | chromosome 16 |
| Location: | 4704146 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687050 | (1 of 8) 2.4.1.5 - Dextransucrase / Sucrose 6-glucosyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGGTCCCTCCAACAATAAGCACCGGCCA |
| Internal bar code: | GGATCCTGTCGGTATACACGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 495 |
| LEAP-Seq percent confirming: | 94.7018 |
| LEAP-Seq n confirming: | 7400 |
| LEAP-Seq n nonconfirming: | 414 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCATGACAGGTTGGTGTC |
| Suggested primer 2: | CTACTGCACTGAGCCCATGA |