Insertion junction: LMJ.RY0402.048231_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.048231
Chromosome:chromosome 6
Location:865837
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g254850 (1 of 1) IPR000008//IPR016024 - C2 domain // Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTATTGTTGTGTGCCTTCCTGTCACGGC
Internal bar code:CAGTACTATAAATTGGAGACTT

Confirmation - LEAP-Seq

LEAP-Seq distance:326
LEAP-Seq percent confirming:99.5501
LEAP-Seq n confirming:7524
LEAP-Seq n nonconfirming:34
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GACGATGATGGTGGGTCTCT
Suggested primer 2:CCATAGAGCGAAGCCTTGTC