| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.048266 |
| Chromosome: | chromosome 5 |
| Location: | 2653618 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236500 | (1 of 1) 3.5.1.23 - Ceramidase / Acylsphingosine deacylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACCCCACCCCGCACACCCCGGGAGGCC |
| Internal bar code: | GGTATTATGGTGCTCCACACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 40 |
| LEAP-Seq percent confirming: | 17.147 |
| LEAP-Seq n confirming: | 119 |
| LEAP-Seq n nonconfirming: | 575 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTGATAAGGCAAGGTCGC |
| Suggested primer 2: | CCCTGAACGGCAATAACAGT |