| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.048275 |
| Chromosome: | chromosome_4 |
| Location: | 2573942 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g222500 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCAAGCCTATGGAGGTGTACCCGATACGGT |
| Internal bar code: | GCCGGATCGAAGGTCGACGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 172 |
| LEAP-Seq percent confirming: | 97.4938 |
| LEAP-Seq n confirming: | 3151 |
| LEAP-Seq n nonconfirming: | 81 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCATGCATTTGACCTTTCC |
| Suggested primer 2: | GATTGGATGGAAAGGAGCAA |