| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.048277 |
| Chromosome: | chromosome 7 |
| Location: | 5691111 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g352500 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTCGGCGAACCCCTACCCCTCGCTCACA |
| Internal bar code: | GGCATCAGACAACGTGAAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 249 |
| LEAP-Seq percent confirming: | 98.5048 |
| LEAP-Seq n confirming: | 3294 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTTACGGCACTTCCTGGC |
| Suggested primer 2: | AAGTACAGCACCTGCTCCGT |