| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.048295 |
| Chromosome: | chromosome 2 |
| Location: | 3378604 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095101 | (1 of 1) PTHR24012:SF459 - RNA-BINDING PROTEIN 4F | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCGCTTTGCGCAGGGAGGGAGTAGCGGC |
| Internal bar code: | CACGTGAATCCCGTAAATTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 969 |
| LEAP-Seq percent confirming: | 97.8824 |
| LEAP-Seq n confirming: | 416 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGACCTACCTGCACCAA |
| Suggested primer 2: | ACGTCAGCATTCCTGCTTCT |