| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.048309 |
| Chromosome: | chromosome 1 |
| Location: | 7994224 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g069472 | TSC2 | Putative organellar cysteinyl tRNA synthetase; (1 of 2) 6.1.1.16 - Cysteine--tRNA ligase / Cysteinyl-tRNA synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCTAGCCCTCTTACACCCAGGGAGGCTT |
| Internal bar code: | AGAAATATCGTTCCTGGACCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 981 |
| LEAP-Seq percent confirming: | 98.2007 |
| LEAP-Seq n confirming: | 1801 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTATCAGGGTGATGGGATG |
| Suggested primer 2: | GTCTCAAGATGAAGGGCTGC |