| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.048323 |
| Chromosome: | chromosome_8 |
| Location: | 4847587 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g384628 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGATGCCTTGCTTTGCTGCCTTGCGTTGC |
| Internal bar code: | GGATATGTGATCGACTGGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 913 |
| LEAP-Seq percent confirming: | 97.6466 |
| LEAP-Seq n confirming: | 27218 |
| LEAP-Seq n nonconfirming: | 656 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATTCCAAAAAGCCCACAG |
| Suggested primer 2: | GGGGCACATGCTCTGTTTAT |