Insertion junction: LMJ.RY0402.048343_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.048343
Chromosome:chromosome 1
Location:603393
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g003487 (1 of 1) K13106 - pre-mRNA-splicing factor CWC26 (BUD13, CWC26) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCGCTCGGCCCCTAGACACGCCACGCA
Internal bar code:GACGTCAACTCTAGCGTTGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:300
LEAP-Seq percent confirming:99.7931
LEAP-Seq n confirming:6754
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CGAACGGTTTGTGTGATACG
Suggested primer 2:CCCCTGCTACTGCTGCTACT