| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.048346 |
| Chromosome: | chromosome 5 |
| Location: | 3218682 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g239900 | ARS15 | Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGAGTCAAAAGCGCAGGGGACAGGATTG |
| Internal bar code: | AGGCAGTCGCGAACGAGGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 784 |
| LEAP-Seq percent confirming: | 93.9547 |
| LEAP-Seq n confirming: | 746 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGCATGTGTGTGTGTGA |
| Suggested primer 2: | ACGTCCTCCCATGCACTTAC |