Insertion junction: LMJ.RY0402.048365_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.048365
Chromosome:chromosome 2
Location:2717735
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g093600 TEH3 (1 of 6) PF03061 - Thioesterase superfamily (4HBT); Thioesterase-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGCGCCCCTCCACCTTCTCCACCTGAGC
Internal bar code:TAGACAGGGCATACGCGAAGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:526
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ACACCCACCTATCCAAGCAG
Suggested primer 2:GGCTTGGCTAGGACTCACTG