| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.048378 |
| Chromosome: | chromosome 12 |
| Location: | 4857081 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g525050 | SMC6A | (1 of 2) PTHR19306//PTHR19306:SF6 - STRUCTURAL MAINTENANCE OF CHROMOSOMES 5,6 SMC5, SMC6 // PROTEIN C23H4.6, ISOFORM A-RELATED; Structural Maintenance of Chromosomes protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGCTTCATAGCCTCGTCCACCAGCGCCT |
| Internal bar code: | AATAAGCGCGCATCATTGTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 734 |
| LEAP-Seq percent confirming: | 97.9715 |
| LEAP-Seq n confirming: | 3912 |
| LEAP-Seq n nonconfirming: | 81 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGTCACACTGATGTAGGC |
| Suggested primer 2: | ATGTGTGTGCGTCTGGGTTA |