Insertion junction: LMJ.RY0402.048414_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.048414
Chromosome:chromosome 17
Location:181325
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g697406 (1 of 45) IPR029052 - Metallo-dependent phosphatase-like 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAAAAAAGGCCCAAAGCCAGCCGTCCCCG
Internal bar code:GTGAATACTCCCGCACACGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:527
LEAP-Seq percent confirming:99.7991
LEAP-Seq n confirming:2980
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGCTCAAGCAGGGTGTAG
Suggested primer 2:CATTCGCAGGATGATGTGAC