| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.048427 |
| Chromosome: | chromosome 9 |
| Location: | 1527084 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397900 | (1 of 1) PTHR21347:SF0 - CLEFT LIP AND PALATE TRANSMEMBRANE PROTEIN 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATTGGACCCTGGTGTGCACTGCACCTT |
| Internal bar code: | CTGGACAATCCGTTGTTACGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 728 |
| LEAP-Seq percent confirming: | 95.2381 |
| LEAP-Seq n confirming: | 860 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCGTTGATAAGTCGTGTT |
| Suggested primer 2: | ATGGAGTAGCCGATCACCAG |