| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.048445 |
| Chromosome: | chromosome 10 |
| Location: | 5005136 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g455400 | RME2 | (1 of 1) 2.1.1.223 - tRNA(1)(Val) (adenine(37)-N(6))-methyltransferase; RNA methylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCGGGGAAGAAGGCGGAACCAGGCTAGA |
| Internal bar code: | CTAAACGCATCGTTAACAGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 288 |
| LEAP-Seq percent confirming: | 99.6315 |
| LEAP-Seq n confirming: | 7029 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATTTAGGTTGGGCTGGTA |
| Suggested primer 2: | TGCCATCTCCACTGCTACTG |