| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.048462 |
| Chromosome: | chromosome 6 |
| Location: | 3597100 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278123 | VPS39 | (1 of 781) IPR000104 - Antifreeze protein, type I; Subunit of VPS-C complex | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGCTCTGCTCTAGCTCTTGGGCTTGAG |
| Internal bar code: | AATATGTGACGTGTCAAGAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 984 |
| LEAP-Seq percent confirming: | 99.4181 |
| LEAP-Seq n confirming: | 3588 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGACAAGGCACATGGTTT |
| Suggested primer 2: | ATCTCCCCGTAACTGCATTG |