| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.048464 |
| Chromosome: | chromosome 9 |
| Location: | 7711453 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g415800 | (1 of 1) K16865 - programmed cell death protein 4 (PDCD4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGTGTATCCTGTACTTGAAGCCTGCAC |
| Internal bar code: | GGGCCGGGAGATTACGAGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 715 |
| LEAP-Seq percent confirming: | 99.2431 |
| LEAP-Seq n confirming: | 2098 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATACGTCCCTTGTCACGCA |
| Suggested primer 2: | CCCGACGTTTTGAGTTGTTT |