| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.048478 |
| Chromosome: | chromosome 6 |
| Location: | 2704256 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g270450 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGCGCCTGAGTGTCTGGACGCATGGAA |
| Internal bar code: | TTATGTCTTTGAATCGGCTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1350 |
| LEAP-Seq percent confirming: | 99.8934 |
| LEAP-Seq n confirming: | 3749 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGCTTACCACACCTCCAT |
| Suggested primer 2: | CTCCCTTGAGGAACTGATGG |