Insertion junction: LMJ.RY0402.048498_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.048498
Chromosome:chromosome 4
Location:2056069
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g219050 SRR10 Scavenger receptor cysteine rich (SRCR) protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGATGTACGTCTTTGTACGACAGCCGGG
Internal bar code:CTGCGACTTAATTCGATTAGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:418
LEAP-Seq percent confirming:99.2908
LEAP-Seq n confirming:280
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TTTAGAACCTCTCCCCACCA
Suggested primer 2:CCGTAACCAGACAAAGCCAT