Insertion junction: LMJ.RY0402.048502_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.048502
Chromosome:chromosome 16
Location:4514414
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g667350 CAV6 (1 of 2) PF00520//PF08016//PF16905 - Ion transport protein (Ion_trans) // Polycystin cation channel (PKD_channel) // Voltage-dependent L-type calcium channel, IQ-associated (GPHH); Voltage-gated Ca2+ channel, alpha subunit 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCTGCACCCTCCGGGACCTCACCAACCT
Internal bar code:CGATGTATTGGCCCGCGGCCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:600
LEAP-Seq percent confirming:98.3581
LEAP-Seq n confirming:5751
LEAP-Seq n nonconfirming:96
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AAATGAGGTGGAGTGTTGGG
Suggested primer 2:CCTCACCCTAGCTTGTCAGC