| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.048503 |
| Chromosome: | chromosome 10 |
| Location: | 2393442 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435300 | AAP1 | Aspartyl aminopeptidase-like protein; (1 of 1) 3.4.11.21 - Aspartyl aminopeptidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCAACGTGGGTCTGGTAGTGCCGTGTG |
| Internal bar code: | AGTAATAGCCGTATATCATTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 208 |
| LEAP-Seq percent confirming: | 42.5 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACCCTGTTGTCACATGC |
| Suggested primer 2: | ATGTAGACCGGGTGGTTCAG |