| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.048504 |
| Chromosome: | chromosome 9 |
| Location: | 2366486 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392000 | (1 of 3) PF09763 - Exocyst complex component Sec3 (Sec3_C) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTTCGATGTAGTTTGGGCTGGTGTCTAC |
| Internal bar code: | AGATGGTAGGTTGCTGCGCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 224 |
| LEAP-Seq percent confirming: | 99.2204 |
| LEAP-Seq n confirming: | 3309 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAAACCTGTGGACCTGAC |
| Suggested primer 2: | CCTCATCTTCCTCCTCCTCC |