| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.048533 |
| Chromosome: | chromosome 17 |
| Location: | 2113022 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g712400 | TERC1,TERC | Thylakoid protein integration factor; (1 of 1) PTHR30238:SF0 - PROTEIN PIGMENT DEFECTIVE 149 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGACCTGCTCTGTCATACAGTCACAGGTA |
| Internal bar code: | CGTCGGGGCGCATCTATAGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 841 |
| LEAP-Seq percent confirming: | 99.695 |
| LEAP-Seq n confirming: | 9805 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGTTGGTGCCGTCGTATT |
| Suggested primer 2: | GCACGGGAGGACAGTGTATT |