| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.048551 |
| Chromosome: | chromosome_2 |
| Location: | 104339 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g074000 | POC18 | Centriole proteome protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGAGGACGATGCAGCAGCGGCCGAGGTGG |
| Internal bar code: | CATACTGTCCAAGACGCGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 964 |
| LEAP-Seq percent confirming: | 94.1429 |
| LEAP-Seq n confirming: | 1318 |
| LEAP-Seq n nonconfirming: | 82 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTTACCACCGCATATGAA |
| Suggested primer 2: | CATGGACTGCACAGTAGCGT |