| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.048584 |
| Chromosome: | chromosome 14 |
| Location: | 1449783 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617850 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTGTGTCCAGTCCTTGACGGTGCAGACT |
| Internal bar code: | TCAGGAACATTATCGGATTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 707 |
| LEAP-Seq percent confirming: | 98.2634 |
| LEAP-Seq n confirming: | 679 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGGACCTAATGTGTGCAA |
| Suggested primer 2: | GCTTACAGAGGGGACACTCG |