| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.048614 |
| Chromosome: | chromosome 12 |
| Location: | 2736035 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g503400 | PRP38 | (1 of 1) K12849 - pre-mRNA-splicing factor 38A (PRPF38A); Pre-mRNA splicing factor, SR-related protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACAAGCCGAAGGAGAAGGCGGCGGCGGG |
| Internal bar code: | GGTGGCAGGGGATGCTCAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 231 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 275 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAACACCTACTGAGGCAC |
| Suggested primer 2: | CCTGGTCACCGTCGTTAGTT |