Insertion junction: LMJ.RY0402.048647_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.048647
Chromosome:chromosome 12
Location:4606351
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g522700 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATGATTTAAGGACATAACAGAAGGACTGG
Internal bar code:GTATGCCCTAAGATCGCGGCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:770
LEAP-Seq percent confirming:99.4574
LEAP-Seq n confirming:2566
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGGGCTACACCGCAACAT
Suggested primer 2:CCTCCTTGAGCCCATTATGA